The Green Tree Frog is found in swampy areas near lakes stream and ponds as well as in some areas in Florida, South Carolina, Maryland, Illinois, Texas, Georgia, and Arkansas. In all of these areas, they can be distinctly heard as they sing to one another. In the wild the main predators for these frogs are birds, fish, snakes and other frogs.

The Green Tree Frog can be held on occasion, and unlike many other frogs it is not poisonous. These frogs should not be overly held, since they can undergo a certain amount of stress this way and become sick or even die because of it. If they are handled carefully and with attention being paid to how they react, they should be fine.

The Green Tree Frog is green in color with white or yellow stripes extending from their jaw all the way down the side of their body. They sometimes have yellow and sometimes orange spots on their back, and sometimes they appear completely green with no stripes or spots visible. The Green Tree Frog has large toe pads that it uses in climbing and jumping. Adult size generally averages between 1.5 to 2.5 inches in length, with females being a little larger than males. Green Tree Frog tadpoles have green bodies with a white stripe from the snout to its eye.

In captivity, the terrarium for a Green Tree Frog should be set up as closely to their natural habitat as possible. Use a minimum 10-gallon glass screen covered set up for one frog. Line the bottom of the terrarium with artificial turf and intricately arrange clean driftwood, sticks and branches throughout. Driftwood should be soaked in a water and bleach mixture for 12 hours and then in pure water for another 12 hours then letting them dry completely and making sure that there are no fumes being emitted from the wood before you place it in the setup. Although some hobbyists may also use gravel as a substrate, this can be problematic because the frogs may inadvertently swallow it when feeding. The same holds true for bark. For this reason other possible substrates include moss (though it can spoil quickly) and coconut fibers. Unless you are using air plants, fake plants are the best setup. Place these throughout the interior of the terrarium as well. Use a heavy water dish with 1 to 2 inches of water in it. Feed these frogs small insects such as crickets. It is recommended that insects be dusted with a calcium powder.

The Green Tree Frog has its breeding season from the end of March until October. The female lays up to 20,000 eggs, which are black and brown, as well as white and are laid on plants floating in the water. The tadpole will grow to a little over 1 inch and has a long tail. The body of the tadpole is green with a light color band on its side.

It has been reported that rain chambers will enhance captive breeding.